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Tools for Blood Collection from Tail
Tools for Blood Collection from Tail
Placing a mouse on a cage lid and grasping the loose skin behind the
Placing a mouse on a cage lid and grasping the loose skin behind the
Push the mouse into the restrainer
Push the mouse into the restrainer
Leave the tail of the mouse outside the cover of the restrainer
Leave the tail of the mouse outside the cover of the restrainer
Amputate the tip of the mouse tail by scissors
Amputate the tip of the mouse tail by scissors
Massage the tail and collect blood by pipetteman
Massage the tail and collect blood by pipetteman
Tools for Blood Collection from Orbital Sinus in Mouse
Tools for Blood Collection from Orbital Sinus in Mouse
Anesthetize a mouse by intraperitoneal injection of Hypnorm
Anesthetize a mouse by intraperitoneal injection of Hypnorm
Use a sharp end glass capillary tube to penetrate the orbital
Use a sharp end glass capillary tube to penetrate the orbital
Collect blood with a vial
Collect blood with a vial
Tools for Cardiac puncture in Mouse
Tools for Cardiac puncture in Mouse
Disinfect the thorax area with 75% alcohol cotton ball
Disinfect the thorax area with 75% alcohol cotton ball
Search for the maximum heart palpitation with your finger
Search for the maximum heart palpitation with your finger
Insert a 24G 1” needle through the thoracic wall at the point of
Insert a 24G 1” needle through the thoracic wall at the point of
Withdraw blood slowly by your right hand
Withdraw blood slowly by your right hand
Tools for blood collection from Saphenous vein in mice
Tools for blood collection from Saphenous vein in mice
Placing a mouse on a cage lid and grasping the loose skin behind the
Placing a mouse on a cage lid and grasping the loose skin behind the
Place the mouse in the restainer
Place the mouse in the restainer
Pull out the leg and removed the hair by a assistant
Pull out the leg and removed the hair by a assistant
Hair can also be shaved by using a small scalpel
Hair can also be shaved by using a small scalpel
The saphenous vein is seen on the surface of the thigh
The saphenous vein is seen on the surface of the thigh
Apply vaseline after disinfect the surface area to reduce clotting and
Apply vaseline after disinfect the surface area to reduce clotting and
Use a 24 G 1” needle to puncture the vein and release blood from the
Use a 24 G 1” needle to puncture the vein and release blood from the
Use a Microvette or a pipetteman with tip to collect blood from the
Use a Microvette or a pipetteman with tip to collect blood from the
Approximate 100 microliters can be collected
Approximate 100 microliters can be collected
Flex the foot of the mouse to reduce the flow of blood back to the
Flex the foot of the mouse to reduce the flow of blood back to the
A cotton ball is applied to the puncture site to stop further bleeding
A cotton ball is applied to the puncture site to stop further bleeding
Tools for Intraperitoneal Injection in Mouse
Tools for Intraperitoneal Injection in Mouse
As soon as the mouse’s head is restrained, the mouse can be picked up
As soon as the mouse’s head is restrained, the mouse can be picked up
Pick up a nude mouse and spin it’s tail to put it in a faint condition
Pick up a nude mouse and spin it’s tail to put it in a faint condition
Grasp the loose skin on the back of the mouse from ears along the legs
Grasp the loose skin on the back of the mouse from ears along the legs
After disinfect the surface area, insert the needle in the lateral
After disinfect the surface area, insert the needle in the lateral
Inject the substance slowly
Inject the substance slowly
A lump of injection substance can be seen through the skin after
A lump of injection substance can be seen through the skin after
Tools for Oral Feeding in Mouse
Tools for Oral Feeding in Mouse
Grasp the loose skin on the back of the mouse and restrain it’s tail
Grasp the loose skin on the back of the mouse and restrain it’s tail
The anatomy picture showed the position of the feeding needle tip
The anatomy picture showed the position of the feeding needle tip
Sexing mice - The distance between the anal and genital orifices is
Sexing mice - The distance between the anal and genital orifices is
Картинки из презентации «Laboratory Animal Handling Technique» к уроку английского языка на тему «Без темы»

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Laboratory Animal Handling Technique

содержание презентации «Laboratory Animal Handling Technique.ppt»
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1Laboratory Animal Handling Technique. 25Placing a mouse on a cage lid and
- Mouse - Rat - Rabbit. grasping the loose skin behind the ears
2Objective. To comply with the Animal with your thumb and forefinger.
Welfare Ordinance and avoid mishandling of 26Place the mouse in the restainer.
animal in research To provide basic 27Pull out the leg and removed the hair
concepts of animal handling technique to by a assistant.
new animal user While offering our concept 28Hair can also be shaved by using a
and techniques to our animal user, we also small scalpel.
encourage comments from experienced animal 29The saphenous vein is seen on the
users. By doing so, we would enrich our surface of the thigh.
knowledge in the field of laboratory 30Apply vaseline after disinfect the
animal research on both sides and further surface area to reduce clotting and
benefit animal welfare as well as the coagulation during blood collection.
credibility of research result in our 31Use a 24 G 1” needle to puncture the
university. vein and release blood from the saphenous
3Laboratory Animal Handling Technique - vein.
Mouse. A. Blood collection from tail vein 32Use a Microvette or a pipetteman with
B. Blood collection from orbital sinus C. tip to collect blood from the saphenous
Blood collection from cardiac puncture D. vein.
Blood collection from saphenous vein E. 33Approximate 100 microliters can be
Intraperitoneal injection F.Subcutaneous collected.
injection G. Oral Feeding H. Sexing. 34Flex the foot of the mouse to reduce
4Blood Collection From Tail in Mouse. the flow of blood back to the puncture
For collection of small amount of blood site.
(Approximate 0.1 ml ). 35A cotton ball is applied to the
5Tools for Blood Collection from Tail. puncture site to stop further bleeding.
75% alcohol cotton ball for surface 36Intraperitoneal Injection in Mouse. A
disinfection Small plastic bottle with 1/2 common method of administering drugs to
cm diameter holes in both ends as mouse rodents.
restrainer Scissors Pipetteman and tips A 37Tools for Intraperitoneal Injection in
vial for blood collection. Mouse. 75% alcohol cotton ball for surface
6Placing a mouse on a cage lid and disinfection 25G 1/2” needle with 1 ml
grasping the loose skin behind the ears by syringe for injection.
the thumb and forefinger. 38Place a mouse on a cage lid and
7Push the mouse into the restrainer. grasping the loose skin behind the ears
8Leave the tail of the mouse outside with your thumb and forefinger.
the cover of the restrainer. 39As soon as the mouse’s head is
9Amputate the tip of the mouse tail by restrained, the mouse can be picked up and
scissors. the tail secured within your ring finger
10Massage the tail and collect blood by and little finger.
pipetteman. 40The injection site should be in the
11Blood Collection From Orbital Sinus in lower left quadrant of the abdomen because
Mouse. Should apply anesthetic before vital organs are absent from this area.
blood withdraw A convenience and easy Only the tip of the needle should
apply method for blood collection in mouse penetrate the abdominal wall to prevent
Collect amount up to 0.5 ml. injection into the intestine.
12Tools for Blood Collection from 41Subcutaneous Injection in Mouse. The
Orbital Sinus in Mouse. 75% alcohol cotton most common method for immunology studies.
ball for surface disinfection Hypnorm for 42Tools for Subcutaneous Injection in
general anesthetic 27 G needle with 1 ml Mouse. 75% alcohol cotton ball for surface
syringe for injection Glass capillary tube disinfection 25G 1 “ needle with 1 ml
and vial for blood collection. syringe for injection.
13Anesthetize a mouse by intraperitoneal 43Pick up a nude mouse and spin it’s
injection of Hypnorm. tail to put it in a faint condition.
14Use a sharp end glass capillary tube 44Grasp the loose skin on the back of
to penetrate the orbital conjunctiva and the mouse from ears along the legs and
rupture the orbital sinus. restrain the legs with your ring finger
15Collect blood with a vial. and little finger.
16Blood Collection From Cardiac Puncture 45After disinfect the surface area,
in Mouse. For collect up to 1 ml of blood insert the needle in the lateral side of
within a short period of time Must be the abdominal wall and push upwards to the
performed under general anesthetic. armpit of the mouse.
17Tools for Cardiac puncture in Mouse. 46Inject the substance slowly.
75% alcohol cotton ball for surface 47A lump of injection substance can be
disinfection Hypnorm used as anesthetic seen through the skin after injection.
27G needle with 1 ml syringe for injection 48Oral Feeding in Mouse. Gastric
24G needle with 3 ml syringe for blood intubation ensures that all the material
withdraw. was administered Feeding amount limited to
18Anesthetize a mouse by intraperitoneal 1% of body weight.
injection of Hypnorm. 49Tools for Oral Feeding in Mouse. A 18
19Disinfect the thorax area with 75% G stainless steel, ball tipped needle a
alcohol cotton ball. glove.
20Search for the maximum heart 50Grasp the loose skin on the back of
palpitation with your finger. the mouse and restrain it’s tail with your
21Insert a 24G 1” needle through the ring finger and little finger. Then,
thoracic wall at the point of maximum introduce the feeding tube from the
heart palpitation. pharynx in to the esophagus when the mouse
22Withdraw blood slowly by your right is in the act of swallowing.
hand. 51Common complications associated with
23Blood Collection From Saphenous Vein gastric intubation are damage to the
in Mouse. This method is used of multiple esophagus and administration of substance
samples are taken in the course of a day into the trachea. Careful and gentle
It can also be applied on rats, hamsters, passage of the feeding needle will greatly
gerbils and guinea-pigs. reduce these possibilities.
24Tools for blood collection from 52The anatomy picture showed the
Saphenous vein in mice. 75% alcohol cotton position of the feeding needle tip inside
ball for surface disinfection 50 ml the esophagus with the heart and sternum
syringe tube with small holes at the end removed.
as restrainer a scalpel and shaver for 53Sexing mice - The distance between the
remove of hair 24 G 1 “ needle for release anal and genital orifices is greater in
of blood tips and pipetteman for blood the male (left) compared to the female
collection. (right).
Laboratory Animal Handling Technique.ppt
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