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Laboratory Animal Handling Technique
Laboratory Animal Handling Technique
Objective
Objective
Laboratory Animal Handling Technique - Mouse
Laboratory Animal Handling Technique - Mouse
Blood Collection From Tail in Mouse
Blood Collection From Tail in Mouse
Tools for Blood Collection from Tail
Tools for Blood Collection from Tail
Placing a mouse on a cage lid and grasping the loose skin behind the
Placing a mouse on a cage lid and grasping the loose skin behind the
Push the mouse into the restrainer
Push the mouse into the restrainer
Leave the tail of the mouse outside the cover of the restrainer
Leave the tail of the mouse outside the cover of the restrainer
Amputate the tip of the mouse tail by scissors
Amputate the tip of the mouse tail by scissors
Massage the tail and collect blood by pipetteman
Massage the tail and collect blood by pipetteman
Blood Collection From Orbital Sinus in Mouse
Blood Collection From Orbital Sinus in Mouse
Tools for Blood Collection from Orbital Sinus in Mouse
Tools for Blood Collection from Orbital Sinus in Mouse
Anesthetize a mouse by intraperitoneal injection of Hypnorm
Anesthetize a mouse by intraperitoneal injection of Hypnorm
Use a sharp end glass capillary tube to penetrate the orbital
Use a sharp end glass capillary tube to penetrate the orbital
Collect blood with a vial
Collect blood with a vial
Blood Collection From Cardiac Puncture in Mouse
Blood Collection From Cardiac Puncture in Mouse
Tools for Cardiac puncture in Mouse
Tools for Cardiac puncture in Mouse
Anesthetize a mouse by intraperitoneal injection of Hypnorm
Anesthetize a mouse by intraperitoneal injection of Hypnorm
Disinfect the thorax area with 75% alcohol cotton ball
Disinfect the thorax area with 75% alcohol cotton ball
Search for the maximum heart palpitation with your finger
Search for the maximum heart palpitation with your finger
Insert a 24G 1” needle through the thoracic wall at the point of
Insert a 24G 1” needle through the thoracic wall at the point of
Withdraw blood slowly by your right hand
Withdraw blood slowly by your right hand
Blood Collection From Saphenous Vein in Mouse
Blood Collection From Saphenous Vein in Mouse
Tools for blood collection from Saphenous vein in mice
Tools for blood collection from Saphenous vein in mice
Placing a mouse on a cage lid and grasping the loose skin behind the
Placing a mouse on a cage lid and grasping the loose skin behind the
Place the mouse in the restainer
Place the mouse in the restainer
Pull out the leg and removed the hair by a assistant
Pull out the leg and removed the hair by a assistant
Hair can also be shaved by using a small scalpel
Hair can also be shaved by using a small scalpel
The saphenous vein is seen on the surface of the thigh
The saphenous vein is seen on the surface of the thigh
Apply vaseline after disinfect the surface area to reduce clotting and
Apply vaseline after disinfect the surface area to reduce clotting and
Use a 24 G 1” needle to puncture the vein and release blood from the
Use a 24 G 1” needle to puncture the vein and release blood from the
Use a Microvette or a pipetteman with tip to collect blood from the
Use a Microvette or a pipetteman with tip to collect blood from the
Approximate 100 microliters can be collected
Approximate 100 microliters can be collected
Flex the foot of the mouse to reduce the flow of blood back to the
Flex the foot of the mouse to reduce the flow of blood back to the
A cotton ball is applied to the puncture site to stop further bleeding
A cotton ball is applied to the puncture site to stop further bleeding
Intraperitoneal Injection in Mouse
Intraperitoneal Injection in Mouse
Tools for Intraperitoneal Injection in Mouse
Tools for Intraperitoneal Injection in Mouse
Place a mouse on a cage lid and grasping the loose skin behind the
Place a mouse on a cage lid and grasping the loose skin behind the
As soon as the mouse’s head is restrained, the mouse can be picked up
As soon as the mouse’s head is restrained, the mouse can be picked up
The injection site should be in the lower left quadrant of the abdomen
The injection site should be in the lower left quadrant of the abdomen
Subcutaneous Injection in Mouse
Subcutaneous Injection in Mouse
Tools for Subcutaneous Injection in Mouse
Tools for Subcutaneous Injection in Mouse
Pick up a nude mouse and spin it’s tail to put it in a faint condition
Pick up a nude mouse and spin it’s tail to put it in a faint condition
Grasp the loose skin on the back of the mouse from ears along the legs
Grasp the loose skin on the back of the mouse from ears along the legs
After disinfect the surface area, insert the needle in the lateral
After disinfect the surface area, insert the needle in the lateral
Inject the substance slowly
Inject the substance slowly
A lump of injection substance can be seen through the skin after
A lump of injection substance can be seen through the skin after
Oral Feeding in Mouse
Oral Feeding in Mouse
Tools for Oral Feeding in Mouse
Tools for Oral Feeding in Mouse
Grasp the loose skin on the back of the mouse and restrain it’s tail
Grasp the loose skin on the back of the mouse and restrain it’s tail
Common complications associated with gastric intubation are damage to
Common complications associated with gastric intubation are damage to
The anatomy picture showed the position of the feeding needle tip
The anatomy picture showed the position of the feeding needle tip
Sexing mice - The distance between the anal and genital orifices is
Sexing mice - The distance between the anal and genital orifices is

Презентация: «Laboratory Animal Handling Technique». Автор: boshan. Файл: «Laboratory Animal Handling Technique.ppt». Размер zip-архива: 7028 КБ.

Laboratory Animal Handling Technique

содержание презентации «Laboratory Animal Handling Technique.ppt»
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1 Laboratory Animal Handling Technique

Laboratory Animal Handling Technique

- Mouse - Rat - Rabbit

2 Objective

Objective

To comply with the Animal Welfare Ordinance and avoid mishandling of animal in research To provide basic concepts of animal handling technique to new animal user While offering our concept and techniques to our animal user, we also encourage comments from experienced animal users. By doing so, we would enrich our knowledge in the field of laboratory animal research on both sides and further benefit animal welfare as well as the credibility of research result in our university

3 Laboratory Animal Handling Technique - Mouse

Laboratory Animal Handling Technique - Mouse

A. Blood collection from tail vein B. Blood collection from orbital sinus C. Blood collection from cardiac puncture D. Blood collection from saphenous vein E. Intraperitoneal injection F.Subcutaneous injection G. Oral Feeding H. Sexing

4 Blood Collection From Tail in Mouse

Blood Collection From Tail in Mouse

For collection of small amount of blood (Approximate 0.1 ml )

5 Tools for Blood Collection from Tail

Tools for Blood Collection from Tail

75% alcohol cotton ball for surface disinfection Small plastic bottle with 1/2 cm diameter holes in both ends as mouse restrainer Scissors Pipetteman and tips A vial for blood collection

6 Placing a mouse on a cage lid and grasping the loose skin behind the

Placing a mouse on a cage lid and grasping the loose skin behind the

ears by the thumb and forefinger

7 Push the mouse into the restrainer

Push the mouse into the restrainer

8 Leave the tail of the mouse outside the cover of the restrainer

Leave the tail of the mouse outside the cover of the restrainer

9 Amputate the tip of the mouse tail by scissors

Amputate the tip of the mouse tail by scissors

10 Massage the tail and collect blood by pipetteman

Massage the tail and collect blood by pipetteman

11 Blood Collection From Orbital Sinus in Mouse

Blood Collection From Orbital Sinus in Mouse

Should apply anesthetic before blood withdraw A convenience and easy apply method for blood collection in mouse Collect amount up to 0.5 ml

12 Tools for Blood Collection from Orbital Sinus in Mouse

Tools for Blood Collection from Orbital Sinus in Mouse

75% alcohol cotton ball for surface disinfection Hypnorm for general anesthetic 27 G needle with 1 ml syringe for injection Glass capillary tube and vial for blood collection

13 Anesthetize a mouse by intraperitoneal injection of Hypnorm

Anesthetize a mouse by intraperitoneal injection of Hypnorm

14 Use a sharp end glass capillary tube to penetrate the orbital

Use a sharp end glass capillary tube to penetrate the orbital

conjunctiva and rupture the orbital sinus

15 Collect blood with a vial

Collect blood with a vial

16 Blood Collection From Cardiac Puncture in Mouse

Blood Collection From Cardiac Puncture in Mouse

For collect up to 1 ml of blood within a short period of time Must be performed under general anesthetic

17 Tools for Cardiac puncture in Mouse

Tools for Cardiac puncture in Mouse

75% alcohol cotton ball for surface disinfection Hypnorm used as anesthetic 27G needle with 1 ml syringe for injection 24G needle with 3 ml syringe for blood withdraw

18 Anesthetize a mouse by intraperitoneal injection of Hypnorm

Anesthetize a mouse by intraperitoneal injection of Hypnorm

19 Disinfect the thorax area with 75% alcohol cotton ball

Disinfect the thorax area with 75% alcohol cotton ball

20 Search for the maximum heart palpitation with your finger

Search for the maximum heart palpitation with your finger

21 Insert a 24G 1” needle through the thoracic wall at the point of

Insert a 24G 1” needle through the thoracic wall at the point of

maximum heart palpitation

22 Withdraw blood slowly by your right hand

Withdraw blood slowly by your right hand

23 Blood Collection From Saphenous Vein in Mouse

Blood Collection From Saphenous Vein in Mouse

This method is used of multiple samples are taken in the course of a day It can also be applied on rats, hamsters, gerbils and guinea-pigs

24 Tools for blood collection from Saphenous vein in mice

Tools for blood collection from Saphenous vein in mice

75% alcohol cotton ball for surface disinfection 50 ml syringe tube with small holes at the end as restrainer a scalpel and shaver for remove of hair 24 G 1 “ needle for release of blood tips and pipetteman for blood collection

25 Placing a mouse on a cage lid and grasping the loose skin behind the

Placing a mouse on a cage lid and grasping the loose skin behind the

ears with your thumb and forefinger

26 Place the mouse in the restainer

Place the mouse in the restainer

27 Pull out the leg and removed the hair by a assistant

Pull out the leg and removed the hair by a assistant

28 Hair can also be shaved by using a small scalpel

Hair can also be shaved by using a small scalpel

29 The saphenous vein is seen on the surface of the thigh

The saphenous vein is seen on the surface of the thigh

30 Apply vaseline after disinfect the surface area to reduce clotting and

Apply vaseline after disinfect the surface area to reduce clotting and

coagulation during blood collection.

31 Use a 24 G 1” needle to puncture the vein and release blood from the

Use a 24 G 1” needle to puncture the vein and release blood from the

saphenous vein

32 Use a Microvette or a pipetteman with tip to collect blood from the

Use a Microvette or a pipetteman with tip to collect blood from the

saphenous vein

33 Approximate 100 microliters can be collected

Approximate 100 microliters can be collected

34 Flex the foot of the mouse to reduce the flow of blood back to the

Flex the foot of the mouse to reduce the flow of blood back to the

puncture site

35 A cotton ball is applied to the puncture site to stop further bleeding

A cotton ball is applied to the puncture site to stop further bleeding

36 Intraperitoneal Injection in Mouse

Intraperitoneal Injection in Mouse

A common method of administering drugs to rodents

37 Tools for Intraperitoneal Injection in Mouse

Tools for Intraperitoneal Injection in Mouse

75% alcohol cotton ball for surface disinfection 25G 1/2” needle with 1 ml syringe for injection

38 Place a mouse on a cage lid and grasping the loose skin behind the

Place a mouse on a cage lid and grasping the loose skin behind the

ears with your thumb and forefinger

39 As soon as the mouse’s head is restrained, the mouse can be picked up

As soon as the mouse’s head is restrained, the mouse can be picked up

and the tail secured within your ring finger and little finger

40 The injection site should be in the lower left quadrant of the abdomen

The injection site should be in the lower left quadrant of the abdomen

because vital organs are absent from this area. Only the tip of the needle should penetrate the abdominal wall to prevent injection into the intestine.

41 Subcutaneous Injection in Mouse

Subcutaneous Injection in Mouse

The most common method for immunology studies

42 Tools for Subcutaneous Injection in Mouse

Tools for Subcutaneous Injection in Mouse

75% alcohol cotton ball for surface disinfection 25G 1 “ needle with 1 ml syringe for injection

43 Pick up a nude mouse and spin it’s tail to put it in a faint condition

Pick up a nude mouse and spin it’s tail to put it in a faint condition

44 Grasp the loose skin on the back of the mouse from ears along the legs

Grasp the loose skin on the back of the mouse from ears along the legs

and restrain the legs with your ring finger and little finger

45 After disinfect the surface area, insert the needle in the lateral

After disinfect the surface area, insert the needle in the lateral

side of the abdominal wall and push upwards to the armpit of the mouse

46 Inject the substance slowly

Inject the substance slowly

47 A lump of injection substance can be seen through the skin after

A lump of injection substance can be seen through the skin after

injection

48 Oral Feeding in Mouse

Oral Feeding in Mouse

Gastric intubation ensures that all the material was administered Feeding amount limited to 1% of body weight

49 Tools for Oral Feeding in Mouse

Tools for Oral Feeding in Mouse

A 18 G stainless steel, ball tipped needle a glove

50 Grasp the loose skin on the back of the mouse and restrain it’s tail

Grasp the loose skin on the back of the mouse and restrain it’s tail

with your ring finger and little finger. Then, introduce the feeding tube from the pharynx in to the esophagus when the mouse is in the act of swallowing.

51 Common complications associated with gastric intubation are damage to

Common complications associated with gastric intubation are damage to

the esophagus and administration of substance into the trachea. Careful and gentle passage of the feeding needle will greatly reduce these possibilities.

52 The anatomy picture showed the position of the feeding needle tip

The anatomy picture showed the position of the feeding needle tip

inside the esophagus with the heart and sternum removed.

53 Sexing mice - The distance between the anal and genital orifices is

Sexing mice - The distance between the anal and genital orifices is

greater in the male (left) compared to the female (right).

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